WebI am not aware of a principle from which one could derive an "appropriate" value, but in practice, on data sets with 50-100 samples, using 0.15 to 0.2 has worked fairly well. For fewer samples a larger valuse (0.25 to 0.3) may be warranted. WebmergeCutHeight “0.25”, “detectCutHeight “0.99995” (code for module construction can be found in Additional file 3). Then, gene expression was summa-rized into module eigengene (ME) as the first principal component (PC) of the entire module gene expression. …
WGCNA_tutorial/WGCNA_tutorial_Rscript at main - Github
Web10 okt. 2024 · WGCNA question - REAL () can only be applied to a 'numeric', not a 'integer'. 01-14-2016, 04:41 AM. Hi, I'm tried to use the WGCNA bioconductor package on my RNAseq data - it was originally integer FPKM counts from the tuxedo pipeline, so I log2 (x+1) transformed it (as was reccomended on the WGCNA bioconductor page), because … Web18 feb. 2024 · Sweet sorghum is a C4 crop that can be grown for silage forage, fiber, syrup and fuel production. It is generally considered a salt-tolerant plant. However, the salt tolerance ability varies among genotypes, and the mechanism is not well known. To further uncover the salt tolerance mechanism, we performed comparative transcriptome … dry bamboo sticks
Bioinformatics analysis of myocardial immune progression
Web1 dag geleden · 1.Introduction. Fish, as the oldest vertebrates, live in almost all aquatic environments. Water temperature is the most important environmental factor for fish, affecting various biological processes, including growth, development, reproduction, and metabolism (Fonds et al., 1992; Handeland et al., 2008; Watson et al., 2024).Most fish … Webnet = blockwiseModules (datExpr0, power = 2, maxBlockSize = 20000, TOMType = "signed", minModuleSize = 30, reassignThreshold = 0, mergeCutHeight = 0.25, numericLabels = TRUE, pamRespectsDendro = FALSE, saveTOMs = TRUE, saveTOMFileBase = "E17.5", verbose = 3) #> Calculating module eigengenes block-wise … Web3 apr. 2024 · To identify modules, function blockwiseConsensusModules was called with the following parameters: softPower=12, deepSplit=3, mergeCutHeight = 0.25. Only the top 2000 variable genes were used. dry bamboo leaves